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Related Experiment Video

Updated: Nov 2, 2025

RNA Isolation from Mouse Ocular Lens Epithelium and Fiber Cell Bulk Masses
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The aging mouse lens transcriptome.

Adam P Faranda1, Mahbubul H Shihan1, Yan Wang1

  • 1Department of Biological Sciences University of Delaware Newark, DE, 19716, USA.

Experimental Eye Research
|June 13, 2021
PubMed
Summary
This summary is machine-generated.

Aging significantly alters the mouse lens transcriptome, affecting cell differentiation, immune response, and mitochondrial function, providing insights into age-related cataract development.

Keywords:
AgingCholesteroComplement pathwaysLensMetabolismPosterior capsular opacificationSenescenceTranscriptome

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Area of Science:

  • Ophthalmology
  • Genomics
  • Molecular Biology

Background:

  • Age is a primary risk factor for age-related cataract (ARC).
  • The impact of aging on the lens transcriptome remains underexplored.
  • Understanding age-related changes in lens cells is crucial for cataract research.

Purpose of the Study:

  • To elucidate the age-dependent changes in the transcriptome of mouse lens epithelial cells (LECs) and lens fiber cells (LFCs).
  • To identify key genes and pathways affected by aging in the lens.
  • To investigate the genetic underpinnings of age-related cataract.

Main Methods:

  • Isolation of LECs and LFCs from young (3-month) and aged (24-month) C57BL/6J mice.
  • RNA sequencing (RNAseq) to profile the transcriptome.
  • Differential gene expression analysis using EdgeR and pathway analysis with Advaita's Ipathway guide.

Main Results:

  • Age-dependent downregulation of lens differentiation markers (e.g., gamma crystallin) in both LECs and LFCs.
  • Altered expression of key transcription factors (Hsf4, Maf, FoxE3) in aged LFCs.
  • Upregulation of immune response, stress response, and cholesterol metabolism genes in aged LECs and LFCs; evidence of mitochondrial stress in aged LFCs.

Conclusions:

  • Aging profoundly impacts the lens transcriptome, affecting cell differentiation and function.
  • Transcriptomic changes in aged lenses may contribute to the pathophysiology of age-related cataract.
  • The study provides a valuable dataset for future research into lens aging and cataract.