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Updated: Nov 2, 2025

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
Published on: April 18, 2019
Zidovudine enhances activity of carbapenems against NDM-1-producing Enterobacteriaceae
Yanmin Hu1, Anthony Coates1,2
1Institute for Infection and Immunity, St George's University of London, London, UK.
Objectives:
To investigate the efficacy of zidovudine in combination with carbapenems against NDM-1-producing Enterobacteriaceae.
Methods:
MICs were determined using the broth microdilution method. The combinatory effects of zidovudine and carbapenems were examined using the chequerboard method and time-kill analysis.
Results:
We found that the NDM-1-producing strains were resistant to all carbapenems tested. FIC index from chequerboard assay demonstrated that zidovudine synergized with carbapenems against all the NDM-1 strains. Time-kill analysis demonstrated significant synergistic activity when a low level of zidovudine was combined with meropenem.
Conclusions:
Zidovudine in combination with carbapenems produced synergistic activity against NDM-1 Enterobacteriaceae strains in vitro.
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