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Related Experiment Videos

Cardiac acetylcholine concentration in the mouse.

A Nomura1, H Yasuda, H Shimono

  • 1Department of Cardiovascular Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.

Life Sciences
|January 1, 1988
PubMed
Summary

Microwave irradiation of live mice is a suitable sacrifice method for measuring cardiac acetylcholine (ACh). This method yielded significantly different ACh concentrations compared to other techniques, with higher levels found in atria than ventricles.

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Area of Science:

  • Cardiovascular Physiology
  • Neurochemistry

Background:

  • Cardiac acetylcholine (ACh) plays a crucial role in heart function.
  • Accurate measurement of cardiac ACh is essential for understanding its physiological roles.
  • Different methods of animal sacrifice and tissue handling can potentially affect ACh levels.

Purpose of the Study:

  • To compare four different methods for measuring cardiac acetylcholine (ACh) in mice.
  • To determine the most suitable sacrifice method for accurate cardiac ACh quantification.
  • To investigate potential differences in ACh concentrations between cardiac atria and ventricles.

Main Methods:

  • Cardiac ACh was measured in mice subjected to four conditions: in vivo microwave irradiation, in vitro microwave irradiation, immediate measurement post-decapitation (non-frozen), and measurement after heart freezing post-decapitation.

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  • Mice were sacrificed using different methods, including microwave irradiation (in vivo and in vitro) and decapitation followed by immediate or frozen tissue processing.
  • Acetylcholine concentrations were quantified in cardiac tissue, with specific comparisons made between atria and ventricles.

    • Main Results:

    • Significant differences in cardiac ACh concentrations were observed between the in vivo microwave irradiation group and the other three groups.
    • Mice irradiated while alive (in vivo) showed significantly higher atrial ACh concentrations (1.70 ± 0.70 nmol/g) compared to ventricular concentrations (1.07 ± 0.30 nmol/g).
    • The in vivo microwave irradiation method demonstrated suitability for cardiac ACh measurement.

    Conclusions:

    • Microwave irradiation of animals while alive is a suitable method for sacrificing mice when the objective is to measure cardiac acetylcholine.
    • The method of sacrifice significantly impacts measured cardiac ACh levels, highlighting the importance of standardized protocols.
    • Cardiac atria exhibit higher acetylcholine concentrations than ventricles in mice subjected to in vivo microwave irradiation.