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ZhiJingSan Inhibits Osteoclastogenesis via Regulating RANKL/NF-κB Signaling Pathway and Ameliorates Bone Erosion in

Yuanyuan Ling1, Jie Yang1, Di Hua1

  • 1Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Frontiers in Pharmacology
|June 14, 2021
PubMed

Insights

ZhiJingSan (ZJS) effectively treats rheumatoid arthritis (RA) bone erosion by inhibiting osteoclast differentiation via the RANKL/NF-κB pathway. This traditional Chinese medicine shows promise for managing RA joint damage.

Area of Science:

  • Rheumatology
  • Immunology
  • Pharmacology

Background:

  • Rheumatoid arthritis (RA) is characterized by bone erosion, leading to joint deformities and disability.
  • Current RA treatments often fail to adequately address bone erosion.
  • ZhiJingSan (ZJS), a traditional Chinese prescription, demonstrates potential anti-rheumatism and analgesic properties.

Purpose of the Study:

  • To evaluate the therapeutic efficacy of ZJS on bone erosion in a murine model of rheumatoid arthritis.
  • To elucidate the underlying molecular mechanisms by which ZJS exerts its therapeutic effects.

Main Methods:

  • A collagen-induced arthritis (CIA) mouse model was used to assess ZJS's effect on RA bone erosion.
  • In vitro studies utilized an osteoclast differentiation model to investigate ZJS's mechanism of action.
  • Key molecular pathways, including RANKL/NF-κB signaling, were analyzed.

Main Results:

  • ZJS administration delayed arthritis onset, reduced joint inflammation, and attenuated bone erosion in CIA mice.
  • ZJS decreased serum levels of TNF-α, IL-6, and anti-collagen II antibodies.
  • ZJS inhibited osteoclast differentiation and expression of cathepsin K and MMP9 by blocking RANKL-induced NF-κB signaling in vitro.

Conclusions:

  • ZJS demonstrates significant therapeutic effects against RA bone erosion.
  • The mechanism involves the inhibition of RANKL/NF-κB-mediated osteoclast differentiation.
  • ZJS represents a promising therapeutic candidate for treating rheumatoid arthritis-associated bone erosion.
Keywords:
NF-κBRANKLZhiJingSanbone erosionosteoclastsrheumatoid arthritis

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