Related Experiment Video
Updated: Nov 2, 2025
08:23
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
4.4K
Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe
Colum Breen1, Robert Pal2, Mark R J Elsegood1
1Department of Chemistry, Loughborough University Epinal Way Loughborough LE11 3TU UK S.J.Butler@lboro.ac.uk.
Chemical Science
|June 14, 2021
Summary
Scientists developed a new luminescent europium(iii) probe for detecting peroxynitrite (ONOO-) in biological samples. This tool enables sensitive, real-time analysis of ONOO- in cells and serum, crucial for understanding its biological roles.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Cell Biology
Background:
- Peroxynitrite (ONOO-) is a reactive oxygen species involved in cellular processes.
- Direct detection of ONOO- is challenging due to its short lifespan and reactivity.
- Understanding ONOO- roles requires sensitive and selective analytical methods.
Purpose of the Study:
- To develop a novel luminescent probe for ONOO- detection.
- To enable real-time and time-resolved analysis of ONOO- in biological systems.
- To demonstrate the probe's utility in monitoring ONOO- production in cellular models.
Main Methods:
- Synthesis of a water-soluble, luminescent europium(iii) probe.
- Utilizing the probe's long luminescence lifetime for time-resolved measurements.
- Detection of ONOO- in human serum, living cells, and biological matrices.
- Monitoring ONOO- production in cell lines treated with cold atmospheric plasma.
Main Results:
- The Eu(iii) probe allows for rapid and sensitive detection of ONOO-.
- Time-resolved measurements effectively circumvent autofluorescence interference.
- The probe successfully monitored ONOO- production in various cell lines.
- Demonstrated utility in analyzing ONOO- generated by clinical cold atmospheric plasma treatment.
Conclusions:
- The developed Eu(iii) probe is a valuable tool for selective and sensitive ONOO- detection.
- This method facilitates real-time analysis of ONOO- in complex biological samples.
- The probe aids in understanding ONOO- involvement in cellular responses and therapeutic applications.
