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Updated: Nov 2, 2025

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Published on: August 17, 2015
Identifying reactive intermediates by mass spectrometry
1Institute for Molecules and Materials, Radboud University Heyendaalseweg 135 6525 AJ Nijmegen The Netherlands jana.roithova@ru.nl.
Abstract:
Development of new reactions requires finding and understanding of novel reaction pathways. In challenging reactions such as C-H activations, these pathways often involve highly reactive intermediates which are the key to our understanding, but difficult to study. Mass spectrometry has a unique sensitivity for detecting low abundant charged species; therefore it is increasingly used for detection of such intermediates in metal catalysed- and organometallic reactions. This perspective shows recent developments in the field of mass spectrometric research of reaction mechanisms with a special focus on going beyond mass-detection. Chapters discuss the advantages of collision-induced dissociation, ion mobility and ion spectroscopy for characterization of structures of the detected intermediates. In addition, we discuss the relationship between the condensed phase chemistry and mass spectrometric detection of species from solution.
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