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Visual Recognition and Detection of Clindamycin by Au@Ag Core-Shell Nanoparticles
Qiuzheng Du1, Ziwei Jing1, Hang Qi2
1Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
ACS Omega
|June 14, 2021
Summary
A new colorimetric sensor using gold-silver core-shell nanoparticles (Au@Ag NPs) enables rapid, visual detection of clindamycin. This simple method accurately quantifies clindamycin in urine samples with high sensitivity.
Area of Science:
- Nanotechnology
- Analytical Chemistry
- Materials Science
Background:
- Clindamycin detection is crucial for therapeutic drug monitoring and diagnostics.
- Existing analytical methods for clindamycin can be complex and time-consuming.
- Development of rapid, sensitive, and cost-effective detection techniques is needed.
Purpose of the Study:
- To develop a novel colorimetric sensor for the quantitative detection of clindamycin.
- To utilize gold-silver core-shell nanoparticles (Au@Ag NPs) for visual detection.
- To assess the sensor's performance in terms of sensitivity, selectivity, and applicability in real samples.
Main Methods:
- Synthesis and characterization of Au@Ag NPs using transmission electron microscopy (TEM) and UV-vis spectrophotometry.
- Colorimetric detection of clindamycin by observing color changes and UV-vis spectral shifts upon interaction with Au@Ag NPs.
- Optimization of detection conditions and validation of the sensor's performance, including limit of detection (LOD) and recovery studies.
- Application of the sensor for clindamycin determination in human urine samples.
Main Results:
- Au@Ag NPs exhibited a distinct color change from yellow to gray-blue in the presence of clindamycin.
- A linear relationship was observed between the absorption ratio (A546/A400) and clindamycin concentration (6.25 × 10⁻⁷ to 7.50 × 10⁻⁶ mol/L).
- The sensor achieved a low LOD of 2.00 × 10⁻⁷ mol/L, high recovery (100.0-102.0%), and good selectivity for clindamycin.
- Successful application of the sensor for clindamycin detection in human urine samples.
Conclusions:
- The developed Au@Ag NPs-based colorimetric sensor offers a simple, rapid, and intuitive method for clindamycin detection.
- The sensor demonstrates high sensitivity, selectivity, and applicability for analyzing clindamycin in biological matrices like urine.
- This approach provides a low-cost visualization analysis method with potential for detecting other analytes.
