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Related Experiment Video

Updated: Nov 2, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

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Published on: May 31, 2021

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Basophil Activation Test for Allergy Diagnosis.

Inmaculada Doña1, Adriana Ariza2, Tahia D Fernández3

  • 1Allergy Unit, Hospital Regional Universitario de Málaga; Allergy Research Group, Instituto de Investigación Biomédica de Málaga-IBIMA; inmadd@hotmail.com.

Journal of Visualized Experiments : Jove
|June 14, 2021
PubMed
Summary

The basophil activation test (BAT) aids in diagnosing allergies by measuring basophil responses. It serves as a valuable complementary tool, especially when other tests are inconclusive or risky.

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Area of Science:

  • Immunology
  • Allergy Diagnostics

Background:

  • The basophil activation test (BAT) is an in vitro diagnostic method for evaluating IgE-mediated allergic reactions.
  • Its role in diagnostic algorithms for food, insect venom, drug allergies, and chronic urticaria is variable and not well-defined.

Purpose of the Study:

  • To assess the utility and performance of the basophil activation test (BAT) in allergy diagnosis.
  • To identify specific clinical scenarios where BAT can be a valuable complementary tool.

Main Methods:

  • BAT measures basophil activation markers (CD63, CD203c) via flow cytometry in response to allergen/drug exposure.
  • Evaluates basophil response to cross-linking IgE activation.

Main Results:

  • BAT can help confirm allergy diagnoses and potentially avoid controlled challenge tests, particularly in severe cases.
  • It is useful when skin tests (ST) yield false positives, when ST or specific IgE (sIgE) reagents are unavailable, or when results conflict with patient history.

Conclusions:

  • BAT is a useful complementary tool for allergy diagnosis, especially in specific clinical situations.
  • Limitations include variable sensitivity (particularly in drug allergy), time constraints for sample analysis, and lack of standardization across laboratories.