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Related Experiment Video

Updated: Nov 2, 2025

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
08:05

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Published on: May 27, 2021

2.8K

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy.

WonMo Ahn1, Bhupesh Singla1, Brendan Marshall2

  • 1Vascular Biology Center, Medical College of Georgia at Augusta University.

Journal of Visualized Experiments : Jove
|June 14, 2021
PubMed
Summary
This summary is machine-generated.

This study details a method for visualizing membrane ruffling and macropinocytosis using scanning electron microscopy (SEM). It provides protocols for cell culture, stimulation, and sample preparation to investigate cellular processes.

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Last Updated: Nov 2, 2025

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Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy

Background:

  • Membrane ruffling involves actin-rich cell surface protrusions.
  • These protrusions can lead to macropinosome formation, internalizing extracellular fluid.
  • Scanning electron microscopy (SEM) is crucial for visualizing these dynamic cellular events.

Purpose of the Study:

  • To provide a detailed protocol for SEM-based visualization and quantification of membrane ruffling and macropinocytosis.
  • To enable investigation into the regulation and physiological roles of macropinocytosis.
  • To facilitate the discovery of novel regulators and inhibitors of membrane ruffling.

Main Methods:

  • Cell culture and in vitro stimulation of membrane ruffling.
  • Sample preparation for SEM, including fixation and dehydration.
  • High-resolution SEM imaging and quantification of cellular structures.

Main Results:

  • A comprehensive protocol for SEM imaging of membrane ruffles and macropinocytic cups.
  • Methods for quantifying membrane ruffling and associated cellular events.
  • Identification of stimulators and inhibitors impacting membrane ruffle formation.

Conclusions:

  • The described SEM method is valuable for studying macropinocytosis.
  • This protocol aids in understanding the physiological and pathological roles of membrane ruffling.
  • It serves as a foundation for discovering new therapeutic targets for macropinocytosis-related processes.