Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

7.1K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.1K
Proteomics01:33

Proteomics

8.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitigating T cell DNA damage during PARP inhibitor treatment enhances antitumor efficacy.

Science translational medicine·2025
Same author

Parallel Analyses by Mass Spectrometry (MS) and Reverse Phase Protein Array (RPPA) Reveal Complementary Proteomic Profiles in Triple-Negative Breast Cancer (TNBC) Patient Tissues and Cell Cultures.

Proteomics·2024
Same author

Twist-1, a novel regulator of hematopoietic stem cell self-renewal and myeloid lineage development.

Stem cells (Dayton, Ohio)·2014
Same author

A 7.81 W 355 nm ultraviolet picosecond laser using La2CaB10O19 as a nonlinear optical crystal.

Optics express·2014
Same author

Population pharmacokinetics of intravenous levofloxacin 500 mg/day dosage in infected patients.

Die Pharmazie·2014
Same author

ACE2 activity was increased in atherosclerotic plaque by losartan: Possible relation to anti-atherosclerosis.

Journal of the renin-angiotensin-aldosterone system : JRAAS·2014

Related Experiment Video

Updated: Nov 2, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.1K

Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment.

Nan Wang1, Xia Li1, Rongshui Wang1

  • 1Fynn Biotechnologies Ltd., Mills Institute for Personalized Cancer Care, Jinan City, Shandong Province, P. R. China.

Biotechnology Journal
|June 14, 2021
PubMed
Summary
This summary is machine-generated.

Spatial proteogenomic technologies offer deep insights into the tumor microenvironment (TME). These advanced tools are revolutionizing cancer research by revealing complex spatial information for better understanding and treatment.

Keywords:
DSPmulti-omicsspatially-resolvedtumor microenvironment TME

More Related Videos

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.4K
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.5K

Related Experiment Videos

Last Updated: Nov 2, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.1K
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.4K
Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.5K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • The tumor microenvironment (TME) is a complex ecosystem critical for cancer progression, metastasis, and treatment resistance.
  • Understanding the TME's heterogeneity is essential for advancing cancer research and developing effective therapies.

Purpose of the Study:

  • To review emerging spatially-resolved high-plex molecular profiling technologies for TME deconvolution.
  • To highlight the practical applications and future directions of these technologies, particularly the GeoMx Digital Spatial Profiler (DSP).

Main Methods:

  • Review of current spatial proteogenomic technologies including 10X Visium, GeoMx DSP, CODEX, Multi-Omyx, IMS, and MIBI.
  • Discussion of emerging platforms like FISSEQ, MERFISH, Slide-seq, and HDST.
  • Elaboration on DSP for spatial proteogenomic profiling, focusing on immuno-oncology applications.

Main Results:

  • Spatially-resolved high-plex molecular profiling technologies provide invaluable spatial and molecular data from the TME.
  • These technologies enable in-depth understanding of TME composition and function.
  • DSP offers unique features for immuno-oncology research, with promising future developments.

Conclusions:

  • Emerging spatial technologies are rapidly advancing our comprehension of the TME.
  • These powerful tools are reshaping the landscape of cancer research and therapeutic strategies.
  • Continued development and application of spatial proteogenomics will be crucial for future cancer breakthroughs.