Peptoniphilus faecalis sp. nov., isolated from swine faeces

Area of Science:

• Microbiology
• Bacterial Taxonomy
• Genomics

Background:

• The genus Peptoniphilus comprises anaerobic, Gram-positive cocci.
• Understanding bacterial diversity in animal feces is crucial for gut microbiome research.

Purpose of the Study:

• To characterize and name a novel bacterial species isolated from swine feces.
• To determine the phylogenetic, physiological, and chemotaxonomic properties of the novel isolate.

Main Methods:

• Isolation and cultivation of bacteria from swine feces.
• 16S rRNA gene sequencing for phylogenetic analysis.
• Whole genome sequencing for G+C content, genome size, and gene analysis.
• Biochemical tests for phenotypic characterization.
• Cellular fatty acid analysis.

Main Results:

• A novel obligately anaerobic, Gram-positive coccus, strain AGMB00490T, was isolated.
• Phylogenetic analysis placed the isolate within the Peptoniphilus genus, closely related to P. gorbachii (97.22% similarity).
• Genome analysis revealed a G+C content of 31.2 mol%, genome size of 2,129,517 bp, with 34 tRNA and 10 rRNA genes.
• Distinct biochemical profiles and major fatty acids (C16:0, C18:1ω9c) were observed.
• Acetic acid was identified as a major metabolic end product.

Conclusions:

• Strain AGMB00490T represents a novel species, Peptoniphilus faecalis sp. nov.
• The proposed type strain is AGMB00490T (=KCTC 15944T=NBRC 114159T).
• This finding contributes to the taxonomic knowledge of the Peptoniphilus genus.