Characterization and biogeochemical implications of dissolved organic matter in aquatic environments

Dissolved organic matter (DOM) is viewed as one of the most chemically active organic substances on earth. It plays vital roles in the fate, bioavailability and toxicity of aquatic exogenous chemical species (e.g., heavy metals, organic pollutants, and nanomaterials). The characteristics of DOM such low concentrations, salt interference and complexity in aquatic environments and limitations of pretreatment for sample preparation and application of characterization techniques severely limit understanding of its nature and environmental roles. This review provides a characterization continuum of aquatic DOM, and demonstrate its biogeochemical implications, enabling in-depth insight into its nature and environmental roles. A synthesis of the effective DOM pretreatment strategies, comprising extraction and fractionation methods, and characterization techniques is presented. Additionally, the biogeochemical dynamics of aquatic DOM and its environmental implications are discussed. The findings indicate the collection of representative DOM samples from water as the first and critical step for characterizing its properties, dynamics, and environmental implications. However, various pretreatment procedures may alter DOM composition and structure, producing highly variable recoveries and even influencing its subsequent characterization. Therefore, complimentary use of various characterization techniques is highly recommended to obtain as much information on DOM as possible, as each characterization technique exhibits various advantages and limitations. Moreover, DOM could markedly change the physical and chemical properties of exogenous chemical species, influencing their transformation and mobility, and finally altering their potential bioavailability and toxicity. Several research gaps to be addressed include the impact of pretreatment on the composition and structure of aquatic DOM, molecular-level structural elucidation for DOM, and assessment of the effects of DOM dynamics on the fate, bioavailability and toxicity of exogenous chemical species.