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Related Concept Videos

The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Updated: Nov 2, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

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CRISPR-based diagnostics for detection of pathogens.

Gargi Bhattacharjee1, Nisarg Gohil1, Navya Lavina Lam2

  • 1Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India.

Progress in Molecular Biology and Translational Science
|June 15, 2021
PubMed
Summary
This summary is machine-generated.

CRISPR technology offers rapid and precise disease diagnosis with high sensitivity and specificity. This review covers CRISPR-based diagnostic platforms for early detection of pathogens and diseases like cancer, highlighting applications, challenges, and future prospects.

Keywords:
CRISPRCas effectorsCas12Cas13Cas9DiagnosticsSHERLOCK

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Molecular assays enhance disease diagnosis with rapid and precise outcomes.
  • CRISPR technology has advanced in vitro diagnostics, enabling sensitive and rapid tools.
  • Understanding CRISPR-Cas systems expands applications for early pathogen and disease detection, including cancer.

Purpose of the Study:

  • To review CRISPR-based diagnostic platforms.
  • To discuss applications of CRISPR technology in disease diagnosis.
  • To explore challenges and future prospects of CRISPR-Cas systems in diagnostics.

Main Methods:

  • Review of CRISPR-based diagnostic platforms.
  • Analysis of CRISPR applications in pathogen and disease detection.
  • Discussion of CRISPR system's allele specificity for diagnostic tool design.

Main Results:

  • CRISPR offers improved sensitivity and specificity in molecular diagnostics.
  • CRISPR-based tools are field-deployable, portable, sensitive, specific, and rapid.
  • CRISPR's allele specificity is key for accurate diagnostic tool development.

Conclusions:

  • CRISPR technology significantly improves disease diagnosis through sensitive and specific molecular assays.
  • CRISPR-based diagnostics hold promise for early detection of various diseases and pathogens.
  • Further development of CRISPR-Cas systems offers promising future prospects for advanced diagnostic tools.