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Related Experiment Video

Updated: Nov 2, 2025

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

Published on: January 18, 2015

Creating cell lines for mimicking diseases.

Edina Poletto¹, Guilherme Baldo¹

¹Gene Therapy Center, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil; Post-Graduate Program in Genetics and Molecular Biology, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Progress in Molecular Biology and Translational Science

|June 15, 2021

Summary

This summary is machine-generated.

Genome editing tools create accurate cell models for disease research. This chapter details methods for generating and characterizing these in vitro models, including CRISPR-Cas9 applications.

Keywords:

CRISPR-Cas9 Cell line Clonal expansion Clonal isolation HEK293 Single cell Transfection

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Area of Science:

Biotechnology
Cell Biology
Genomics

Background:

Cell lines serve as valuable models for studying diseases and physiological processes.
They are crucial for creating disease models when primary cultures are unavailable.
Advancements in genome editing offer powerful tools for in vitro disease modeling.

Purpose of the Study:

To outline techniques for generating genome-edited cell lines.
To discuss methods for characterizing the phenotypes of these edited cells.
To provide examples of in vitro disease models created using CRISPR-Cas9.

Main Methods:

Selection of appropriate cell lines for editing.
Transfection methods for introducing gene editing tools.
Utilizing available gene editing technologies.
Phenotype characterization techniques.
Application of CRISPR-Cas9 for disease modeling.

Main Results:

Demonstration of techniques for obtaining genome-edited cell lines.
Successful characterization of cellular phenotypes in edited models.
Examples illustrating the creation of in vitro disease models.

Conclusions:

Genome editing, particularly CRISPR-Cas9, enables the creation of precise in vitro disease models.
These models are essential for advancing our understanding of disease mechanisms.
The described techniques facilitate the development of novel research tools.