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Related Experiment Video

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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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MiR-3614-5p Is a Potential Novel Biomarker for Colorectal Cancer.

Lin Han1, Yanjun Sun2, Cansheng Lu3

  • 1Graduate School, Anhui University of Traditional Chinese Medicine, Hefei, China.

Frontiers in Genetics
|June 15, 2021
PubMed
Summary

Low expression of microRNA-3614-5p (miR-3614-5p) is linked to poorer survival in colorectal cancer patients. This microRNA may serve as a prognostic marker, potentially affecting the P53 and P38MAPK pathways.

Keywords:
GSEAMiR-3614-5pTCGAcolorectal cancerprognosis

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-3614-5p (miR-3614-5p) has been detected in various cancers, including colorectal cancer (CRC).
  • The specific role and association of miR-3614-5p in CRC progression and patient outcomes remain largely undefined.

Purpose of the Study:

  • To investigate the relationship between miR-3614-5p expression and colorectal cancer.
  • To evaluate the prognostic value of miR-3614-5p in colorectal cancer patients.
  • To explore the biological functions and pathways associated with miR-3614-5p in CRC.

Main Methods:

  • Utilized the Cancer Genome Atlas (TCGA) database for analysis.
  • Employed Wilcoxon rank-sum test for expression comparison, logistic regression for clinicopathological correlation.
  • Applied Kaplan-Meier and Cox regression for survival analysis, and Gene Set Enrichment Analysis (GSEA) for functional investigation.

Main Results:

  • Decreased miR-3614-5p expression significantly correlated with advanced N stage, M stage, pathologic stage, rectum adenocarcinoma, and lymphatic invasion (p < 0.05).
  • Low miR-3614-5p expression was associated with poorer overall survival (OS) in both univariate (HR=0.599) and multivariate (HR=0.630) analyses (p=0.005 and p=0.021, respectively).
  • GSEA indicated that high miR-3614-5p expression enriches the P53 pathway and enhances immune responses, including NK T cell activation.

Conclusions:

  • MiR-3614-5p expression is significantly altered in colorectal cancer and is associated with adverse clinicopathological features.
  • Reduced miR-3614-5p levels serve as an independent prognostic marker for poor survival in colorectal cancer patients.
  • The P53 and P38MAPK pathways are likely key regulatory pathways influenced by miR-3614-5p in colorectal cancer.