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Related Experiment Video

Updated: Nov 2, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

22.5K

Identification of ACE2 modifiers by CRISPR screening.

Emily J Sherman, Carmen Mirabelli, Vi T Tang

    Biorxiv : the Preprint Server for Biology
    |June 15, 2021
    PubMed
    Summary

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    Researchers identified 35 genes that modify the abundance of Angiotensin-Converting Enzyme 2 (ACE2) on cell surfaces. This discovery clarifies host factors influencing SARS-CoV-2 entry and suggests new therapeutic targets.

    Area of Science:

    • Virology
    • Genetics
    • Molecular Biology

    Background:

    • SARS-CoV-2 viral entry depends on the spike protein binding to the ACE2 receptor on host cells.
    • Understanding the regulation of ACE2 surface expression is crucial for comprehending viral infectivity and developing interventions.

    Approach:

    • High-throughput CRISPR screens were employed to identify genes functionally modulating ACE2 surface abundance in HuH7 cells.
    • 35 genes impacting ACE2 levels were identified, including transcription factors and epigenetic regulators.

    Key Points:

    • Disruption of specific genes like SMAD4, EP300, PIAS1, and BAMBI affects ACE2 at the mRNA level.
    • These genetic modifications influence cellular susceptibility to SARS-CoV-2 infection.
    • Identified regulators are enriched in known transcription factor and epigenetic pathways.

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    Last Updated: Nov 2, 2025

    Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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    Pooled CRISPR-Based Genetic Screens in Mammalian Cells

    Published on: September 4, 2019

    22.5K
    Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
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    Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

    Published on: August 2, 2018

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    Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
    08:49

    Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

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    Conclusions:

    • This study elucidates key host factors governing ACE2 expression and SARS-CoV-2 entry.
    • The identified genes represent potential therapeutic targets for mitigating SARS-CoV-2 infection.