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Related Experiment Video

Updated: Nov 2, 2025

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

Enhanced NRF2 expression mitigates the decline in neural stem cell function during aging.

Annadurai Anandhan^1,2, Konner R Kirwan³, Mandi J Corenblum¹

¹Department of Neurology, University of Arizona, Tucson, AZ, USA.

|June 15, 2021

Summary

This summary is machine-generated.

Augmenting NRF2 (nuclear factor erythroid 2-related factor 2) expression before middle age improved neural stem progenitor cell (NSPC) function and behavior. However, increasing NRF2 after this critical period showed no benefits.

Keywords:

NRF2 aging neural stem cells redox subventricular zone

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Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

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Published on: March 14, 2015

Area of Science:

Neuroscience
Aging Research
Stem Cell Biology

Background:

Aging significantly impacts neural stem progenitor cell (NSPC) biology, but the exact mechanisms remain unclear.
Previous research identified a critical period (CP) of NSPC decline during middle age, linked to reduced NRF2 (nuclear factor erythroid 2-related factor 2) expression.

Purpose of the Study:

To investigate if enhancing NRF2 expression can counteract the age-related decline in NSPC activity during the CP.
To determine the time-dependency of NRF2 modulation for maintaining NSPC function.

Main Methods:

NRF2 expression in rat subventricular zone (SVZ) NSPCs was upregulated using adeno-associated viral vectors (AAV-NRF2-eGFP).
Control vectors (AAV-eGFP) were used for comparison.
Vectors were administered before (11 months) or after (20 months) the identified CP.

Main Results:

Upregulating NRF2 before the CP (at 11 months) significantly improved behavioral functions, including olfactory discrimination and motor tasks.
NRF2 upregulation enhanced NSPC proliferation, self-renewal, neurogenesis, and migration to the olfactory bulb.
Increasing NRF2 after the CP (at 20 months) did not yield significant improvements in cellular or behavioral outcomes.

Conclusions:

NRF2 pathway modulation can support age-related NSPC function.
Activating NRF2 is time-dependent, with interventions being effective only before the critical period of decline.