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Quantitative Temporal Viromics.

Alice Fletcher-Etherington1, Michael P Weekes1

  • 1Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom;

Annual Review of Virology
|June 15, 2021
PubMed
Summary

Quantitative temporal viromics (QTV) uses mass spectrometry to track dynamic changes in host and virus proteins during infection. This technique is vital for understanding viral replication, disease, and developing new antiviral therapies and vaccines.

Keywords:
host microbial interactionsinnate immunityproteomicsviral proteinsviruses

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Area of Science:

  • Virology
  • Proteomics
  • Infectious Diseases

Background:

  • Viral infections cause dynamic changes in host and viral proteins.
  • Understanding these proteomic shifts is crucial for disease research and therapeutic development.
  • Previous methods lacked comprehensive temporal analysis of viral infections.

Purpose of the Study:

  • To review the utility and applications of quantitative temporal viromics (QTV).
  • To highlight how QTV enables detailed study of host-virus protein dynamics over time.
  • To showcase QTV's role in advancing antiviral and vaccine research.

Main Methods:

  • Quantitative temporal viromics (QTV) utilizes mass spectrometry.
  • Isobaric tandem mass tags (TMTs) enable precise quantification of proteomes.
  • QTV allows comparative analysis of host and viral proteins throughout infection time courses.

Main Results:

  • QTV provides precise quantification of host and viral proteomes.
  • It enables dynamic tracking of protein abundance, localization, modifications, and interactions.
  • Numerous studies have successfully applied QTV to various viral infections.

Conclusions:

  • QTV is a powerful technique for studying viral infections comprehensively.
  • It significantly contributes to understanding viral replication and pathogenesis.
  • QTV aids in the development of novel antiviral therapeutics and vaccines.