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Updated: Nov 2, 2025

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
Published on: October 31, 2012
Generation of an induced pluripotent stem cell line (UNCCi002-A) from a healthy donor using a non-integration system
Alvaro A Beltran1, Sarahi G Molina2, Ariana Marquez2
1UNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
Abstract:
The generation of induced pluripotent stem cells (iPSCs) from healthy individuals is an invaluable resource as reference control in disease modeling and drug discovery. This paper details the reprogramming of peripheral blood mononuclear cells (PBMCs) isolated from a healthy 27 years-old male using non-integration technology. The derived iPSCs displayed typical pluripotent stem cell morphology, the capacity to differentiate into the three germ layers, and normal karyotype. This iPSC line will be used as a reference control to study the Cerebral Cavernous Malformation disease mechanism.
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