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MicroRNA-17-5p inhibits thyroid cancer progression by suppressing Early growth response 2 (EGR2)

Xiang Geng1, YangYang Sun2, JinJin Fu3

  • 1Department of Thyroid and Breast Surgery, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, China.

Bioengineered
|June 16, 2021
PubMed

Insights

MicroRNA-17-5p (miR-17-5p) acts as an oncomiR in thyroid cancer, promoting tumor growth by suppressing Early Growth Response 2 (EGR2). Inhibiting miR-17-5p halts cancer progression and tumorigenesis.

Area of Science:

  • Molecular Oncology
  • Cancer Biology

Background:

  • MicroRNAs (miRNAs) are key regulators in tumorigenesis.
  • miR-17-5p is implicated in various cancer progressions.
  • The role of miR-17-5p in thyroid cancer (TC) requires further elucidation.

Purpose of the Study:

  • To investigate the function and mechanism of miR-17-5p in thyroid cancer.
  • To determine the relationship between miR-17-5p and Early Growth Response 2 (EGR2) in TC.

Main Methods:

  • Quantitative reverse transcription PCR (RT-qPCR) for gene expression analysis.
  • Cell proliferation, migration, and invasion assays (CCK8, colony formation, wound-healing, Transwell).
  • Western blot, Dual-Luciferase assay, and Xenograft animal models for mechanistic and in vivo studies.

Main Results:

  • miR-17-5p expression was significantly upregulated in TC tissues and cells.
  • Inhibition of miR-17-5p suppressed TC cell proliferation, migration, invasion, and in vivo tumorigenesis.
  • miR-17-5p negatively regulated EGR2, which was downregulated in TC.
  • EGR2 overexpression inhibited TC development, and its knockdown abrogated the anti-cancer effects of miR-17-5p inhibition.

Conclusions:

  • miR-17-5p functions as a thyroid cancer oncomiR by modulating EGR2.
  • Targeting the miR-17-5p/EGR2 axis presents a potential therapeutic strategy for thyroid cancer.
Keywords:
EGR2metastasismiR-17-5pprogressionthyroid cancer

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