Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of the Platelet Plug01:22

Formation of the Platelet Plug

7.9K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Indirect presentation of mismatched human leukocyte antigen-B associates with outcomes of cord blood transplantation.

British journal of haematology·2025
Same author

STAT1 regulates immune-mediated intestinal stem cell proliferation and epithelial regeneration.

Nature communications·2025
Same author

TIM-3 marks measurable residual leukemic stem cells responsible for relapse after allogeneic stem cell transplantation.

Cancer science·2024
Same author

Clinical significance of NOTCH1 and FBXW7 alterations in adult T-cell leukemia/lymphoma.

International journal of hematology·2024
Same author

A phase 2 clinical trial of luspatercept in non-transfusion-dependent patients with myelodysplastic syndromes.

International journal of hematology·2024
Same author

Allogeneic Hematopoietic Stem Cell Transplantation for Acute Myeloid Leukemia With a Germline <i>DDX41</i> Mutation.

Case reports in hematology·2024

Related Experiment Video

Updated: Nov 2, 2025

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
14:38

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Published on: January 4, 2019

18.7K

Platelet decrease and efficacy of platelet-rich plasma return following peripheral blood stem cell apheresis.

Takahiro Shima1,2, Teppei Sakoda1,2, Tomoko Henzan2

  • 1Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medicine, Fukuoka, Japan.

Journal of Clinical Apheresis
|June 16, 2021
PubMed
Summary

Platelet loss after peripheral blood stem cell (PBSC) apheresis can occur, particularly in autologous settings. Platelet-rich plasma (PRP) return effectively aids platelet recovery post-PBSC apheresis.

Keywords:
PRP returnharvestplatelet loss

More Related Videos

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
08:34

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Published on: February 24, 2021

2.7K
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

20.1K

Related Experiment Videos

Last Updated: Nov 2, 2025

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
14:38

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Published on: January 4, 2019

18.7K
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
08:34

Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion

Published on: February 24, 2021

2.7K
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

20.1K

Area of Science:

  • Hematology
  • Transplantation Medicine
  • Apheresis Technology

Background:

  • Peripheral blood stem cell (PBSC) transplantation is a critical treatment for hematological diseases.
  • Platelet loss is a common complication during PBSC apheresis procedures.
  • The effectiveness of platelet-rich plasma (PRP) return for mitigating platelet loss requires further investigation.

Purpose of the Study:

  • To investigate the frequency and severity of platelet count changes following PBSC apheresis.
  • To evaluate the efficacy of platelet-rich plasma (PRP) return in facilitating platelet recovery post-apheresis.
  • To identify risk factors for severe platelet decrease after autologous PBSC apheresis.

Main Methods:

  • Assessed platelet count changes in 270 allogeneic and 105 autologous PBSC apheresis settings.
  • Evaluated the impact of platelet-rich plasma (PRP) transfusion on post-apheresis platelet recovery.
  • Analyzed pre-apheresis platelet count as a predictor of severe post-apheresis thrombocytopenia.

Main Results:

  • Platelet counts decreased post-apheresis in both allogeneic and autologous settings.
  • Severe platelet decrease (<50 × 10⁹/L) was observed in 9 autologous PBSC patients.
  • Pre-apheresis platelet count was a significant risk factor for severe platelet decrease in autologous PBSC apheresis (OR 0.749, P < .049).
  • Platelet-rich plasma (PRP) return successfully facilitated platelet recovery in over 80% of cases in both settings.

Conclusions:

  • A lower pre-apheresis platelet count predicts severe platelet decrease after autologous PBSC apheresis.
  • Platelet-rich plasma (PRP) return is an effective intervention for enhancing platelet recovery post-apheresis.
  • These findings support the use of PRP return to manage thrombocytopenia following PBSC apheresis.