Related Experiment Video
Updated: Nov 2, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Redox feedback regulation of ANAC089 signaling alters seed germination and stress response
Pablo Albertos1, Kiyoshi Tatematsu2, Isabel Mateos1
1Department of Botany and Plant Physiology, Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), Facultad de Biología, Universidad de Salamanca, C/Río Duero 12, 37185 Salamanca, Spain.
Abstract:
The interplay between the phytohormone abscisic acid (ABA) and the gasotransmitter nitric oxide (NO) regulates seed germination and post-germinative seedling growth. We show that GAP1 (germination in ABA and cPTIO 1) encodes the transcription factor ANAC089 with a critical membrane-bound domain and extranuclear localization. ANAC089 mutants lacking the membrane-tethered domain display insensitivity to ABA, salt, and osmotic and cold stresses, revealing a repressor function. Whole-genome transcriptional profiling and DNA-binding specificity reveals that ANAC089 regulates ABA- and redox-related genes. ANAC089 truncated mutants exhibit higher NO and lower ROS and ABA endogenous levels, alongside an altered thiol and disulfide homeostasis. Consistently, translocation of ANAC089 to the nucleus is directed by changes in cellular redox status after treatments with NO scavengers and redox-related compounds. Our results reveal ANAC089 to be a master regulator modulating redox homeostasis and NO levels, able to repress ABA synthesis and signaling during Arabidopsis seed germination and abiotic stress.
More Related Videos
Related Concept Videos
Cell Signaling in Plants
Gene Regulation During Sporulation
Responses to Heat and Cold Stress
Photoreceptors and Plant Responses to Light
Regulation of Transpiration by Stomata
Global Regulatory Systems

