A reference map of sphingolipids in murine tissues.

Area of Science:

• Lipidomics
• Biochemistry
• Systems Biology

Background:

• Sphingolipids (SPs) are crucial for cell membrane structure and signaling.
• Their structural diversity and low abundance present identification and quantification challenges.
• Advances in lipidomics, particularly LC-MS, enable reliable detection and quantification.

Purpose of the Study:

• To create a comprehensive
• murine sphingolipid atlas
• using quantitative lipidomics.
• To map the qualitative and quantitative distribution of 114 SPs in 21 distinct tissues.
• To identify tissue-specific and sex-specific sphingolipid profiles.

Main Methods:

• Utilized liquid chromatography coupled with mass spectrometry (LC-MS).
• Analyzed sphingolipid content in 21 tissues from C57BL/6 mice.
• Compiled a quantitative atlas of 114 sphingolipid species.

Main Results:

• Established a detailed sphingolipidomic map of murine tissues.
• Reported 114 sphingolipids with qualitative and quantitative distribution.
• Identified distinct tissue-specific sphingolipid fingerprints.
• Observed significant sex-specific differences in sphingolipid profiles within tissues.

Conclusions:

• The murine sphingolipid atlas provides a comprehensive quantitative resource.
• This atlas will aid in investigating sphingolipid roles in mammalian health and disease.
• The data complements existing tissue compendia for broader biological insights.