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Proteomic analysis of an Enterococcus faecalis mutant generated against the exposure to silver nanoparticles
Marco Felipe Salas-Orozco1, Nereyda Niño-Martínez2, Gabriel-Alejandro Martínez-Castañón2
1Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Introduction:
Nanoparticles (NPs) have been widely studied as an alternative to antibiotic use due to their antimicrobial properties at lower concentrations. Enterococcus faecalis is a facultative Gram-positive microorganism inhabiting the gastrointestinal tract of humans and animals. It can also be present in other environments such as the oral cavity, water, sewage, soil and food.
Aims:
We evaluated whether E. faecalis could develop resistance to silver NPs (AgNPs) after exposure to sublethal concentrations of the NPs.
Methods And Results:
Proteomic analyses revealed that different pathways were activated during the acquired resistance under sublethal concentrations, and selected genes were validated by qPCR.
Conclusions:
The results of this study showed that E. faecalis is capable of generating resistance to AgNPs.
Significance And Impact Of The Study:
To avoid the generation of resistance against AgNPs, future use of these NPs should be combined with other NPs prepared with different metals to prevent the dissemination of resistant strains.
