Geometrically regulating evolutionary dynamics in biofilms

The theoretical understanding of evolutionary dynamics in spatially structured populations often relies on nonspatial models. Biofilms are among such populations where a more accurate understanding is of theoretical interest and can reveal new solutions to existing challenges. Here, we studied how the geometry of the environment affects the evolutionary dynamics of expanding populations, using the Eden model. Our results show that fluctuations of subpopulations during range expansion in two- and three-dimensional environments are not Brownian. Furthermore, we found that the substrate's geometry interferes with the evolutionary dynamics of populations that grow upon it. Inspired by these findings, we propose a periodically wedged pattern on surfaces prone to develop biofilms. On such patterned surfaces, natural selection becomes less effective and beneficial mutants would have a harder time establishing. Additionally, this modification accelerates genetic drift and leads to less diverse biofilms. Both interventions are highly desired for biofilms.