Seasonal and areal variability in PM_2.5 poses differential degranulation and pro-inflammatory effects on RBL-2H3...

PM_2.5 pollution is a widespread environmental and health problem, particularly in China. Besides leading to well-known diseases in the respiratory system, PM_2.5 can also alter immune function to induce or aggravate allergic diseases. To determine whether there are temporal and spatial differences in the allergic responses to PM_2.5, monthly samples were collected from four regions (urban, industrial, suburban, and rural areas) through a whole year in Nanjing city, China. Inorganic chemical components (metals and water-soluble ions) of PM_2.5 were analyzed, and the rat basophil cells (RBL-2H3) exposed to PM_2.5 were assessed through quantitative measures of degranulation (β-hex and histamine) and pro-inflammation cytokine (IL-4 and TNF-α) expression. The highest levels of β-hex were measured in winter and spring PM_2.5 from urban and industrial areas, or autumn PM_2.5 from suburban and rural areas. With respect to histamine, autumn PM_2.5 samples were most potent irrespective of the location. Autumn and winter PM_2.5 induced higher levels of IL-4 than spring and summer samples. However, spring and autumn PM_2.5 caused higher levels of TNF-α. The concentrations of water-soluble ions (NH₄⁺, K⁺ and Cl^-), as well as heavy metals (Pb and Cr), were directly and statistically correlated to the inflammation observed in vitro. In general, the differences between regional and seasonal PM_2.5 in stimulating cell degranulation may depend on endotoxin and airborne allergen content of PM_2.5. The heavy metals and water-soluble ions in PM_2.5 were mostly anthropogenic, which increased the particles' mass-based cellular inflammatory potential, therefore, their health risks, e.g. from vehicular exhaust, coal, and biomass combustion, cannot be ignored.