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Related Concept Videos

Antibody Actions01:26

Antibody Actions

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Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
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Conjugated Proteins02:50

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Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
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Related Experiment Video

Updated: Nov 2, 2025

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Multivalency transforms SARS-CoV-2 antibodies into ultrapotent neutralizers.

Edurne Rujas1,2,3, Iga Kucharska1, Yong Zi Tan1

  • 1Program in Molecular Medicine, The Hospital for Sick Children Research Institute, Toronto, ON, Canada.

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|June 17, 2021
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Researchers developed a novel Multibody platform using human apoferritin to create highly potent neutralizing antibodies against SARS-CoV-2. This approach significantly enhances antibody efficacy, offering a promising therapeutic strategy for COVID-19 and other viral diseases.

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Area of Science:

  • Biotechnology
  • Immunology
  • Virology

Background:

  • Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) causes the COVID-19 pandemic.
  • Antibodies are effective biotherapeutics against viral infections.
  • Existing antibody therapies face challenges in potency and broad efficacy.

Purpose of the Study:

  • To engineer ultrapotent neutralizing antibodies against SARS-CoV-2.
  • To develop a modular platform for creating multivalent and multispecific antibody therapeutics.
  • To enhance antibody potency and overcome viral sequence variability.

Main Methods:

  • Utilized human apoferritin protomers to drive antibody fragment oligomerization.
  • Constructed multivalent molecules combining multiple antibody specificities and Fc domains.
  • Developed the MULTi-specific, multi-Affinity antiBODY (Multabody or MB) platform.

Main Results:

  • Achieved half-maximal inhibitory concentration (IC50) values as low as 9 × 10^-14 M.
  • Demonstrated up to 10,000-fold potency enhancement compared to immunoglobulin G (IgG).
  • Created molecules with broad efficacy against viral sequence variability and IgG-like bioavailability.

Conclusions:

  • The Multabody platform delivers ultrapotent and broad-spectrum SARS-CoV-2 neutralizers.
  • The platform's modularity allows rapid adaptation for other infectious diseases.
  • This approach represents a significant advancement in developing antibody-based therapeutics.