Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Endotoxemia and cholestasis.

P T Roughneen¹, S C Kumar, N R Pellis

¹Department of Surgery, University of Texas Medical School, Houston.

Surgery, Gynecology & Obstetrics

|September 1, 1988

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mucins and CD56 as markers of tumour invasion and prognosis in periampullary cancer.

The British journal of surgery·2010

Same author

Novel substrates to maintain gut integrity.

Nutrition research reviews·2008

Same author

Quantitative evaluation of europium in blue ballpoint pen inks/offset printing inks tagged with europium thenoyltrifluoroacetonate by spectrofluorometry and ICP-AES.

Science & justice : journal of the Forensic Science Society·2008

Same author

The morphine-prostigmine provocation (Nardi) test for sphincter of Oddi dysfunction: results in healthy volunteers and in patients before and after transduodenal sphincteroplasty and transampullary septectomy.

Gut·2007

Same author

The effect of jaundice on the generation of anti-gastrin antibodies in G17DT immunized patients with advanced pancreatic cancer.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2005

Same author

The use of ultra filtration in trace metal speciation studies in sea water.

Environment international·2005

Same journal

THE USE OF COMBINED PRESERVATION TECHNIQUES FOR EXTENDED STORAGE OF ORTHOTOPIC LIVER HOMOGRAFTS.

Surgery, gynecology & obstetrics·2010

Same journal

The Role of Electrical Pacemakers in the Treatment of Unexpected Cardiac Arrest.

Surgery, gynecology & obstetrics·2010

Same journal

Colonic and proctoscopic diseases.

Surgery, gynecology & obstetrics·2010

Same journal

A summary of the surgical aspects of certain sulfonamides and antibiotic agents.

Surgery, gynecology & obstetrics·2010

Same journal

Penetrating cranial wounds; a summary of methods used in management; collective review.

Surgery, gynecology & obstetrics·2010

Same journal

The use of compression in the treatment of injuries.

Surgery, gynecology & obstetrics·2010

See all related articles

Obstructive jaundice may not cause endotoxemia by increasing gut endotoxin absorption due to reduced bile salt flow. This study found no evidence supporting this common theory in rats, suggesting other causes for endotoxemia.

Area of Science:

Gastroenterology
Hepatology
Microbiology

Background:

Endotoxemia is a significant factor in obstructive jaundice complications.
A prevailing hypothesis suggests reduced bile salt flow in cholestasis increases intestinal endotoxin absorption, leading to endotoxemia.

Purpose of the Study:

To investigate the role of gastrointestinal bile salt flow in the pathogenesis of endotoxemia in obstructive jaundice.
To re-evaluate the hypothesis linking cholestasis, bile salt deficiency, and enhanced endotoxin absorption.

Main Methods:

Utilized female Sprague-Dawley rats with bile duct ligation or sham operations.
Employed quantitative chromogenic and qualitative Limulus assays for bacterial endotoxin detection.
Measured serum total bilirubin, serum bile acid, and intestinal bile acid concentrations at various time points post-operation.

Related Experiment Videos

Main Results:

Bile duct ligation led to elevated serum bilirubin and bile acids, and decreased intestinal bile acids.
Despite reduced gastrointestinal bile salt flow, quantitative and qualitative Limulus assays did not detect increased portal or systemic endotoxemia.
No correlation was found between cholestasis and increased endotoxin absorption.

Conclusions:

The study refutes the hypothesis that diminished gastrointestinal bile salt flow enhances intestinal endotoxin absorption in obstructive jaundice.
Alternative mechanisms are likely responsible for endotoxemia in patients with obstructive jaundice.
Findings challenge established pathophysiological concepts in cholestasis-related endotoxemia.