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Updated: Nov 1, 2025

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Published on: March 9, 2021
Detection of parallel and antiparallel DNA triplex structures in living human cells using in-cell NMR
Tomoki Sakamoto1, Yudai Yamaoki1, Takashi Nagata1
1Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. katahira.masato.6u@kyoto-u.ac.jp and Graduate School of Energy Science, Kyoto University, Yoshida-hommachi, Sakyo-ku, Kyoto 606-8501, Japan.
Abstract:
We introduced oligodeoxynucleotides (ODNs) that form parallel and antiparallel triplex structures in vitro into living human cells and recorded their in-cell NMR spectra. Observation of landmark signals for triplex structures proved for the first time that parallel and antiparallel triplex structures are formed in living human cells.

