Lead-induced changes in the stabilization of the mouse sperm chromatin

Long-term exposure of male mice to inorganic lead was previously found to reduce their fertility. In the present study chromatin stability in spermatozoa from such mice was investigated by means of quantitative cytochemical analysis. Sperm head sizes were determined and the capacity for nuclear chromatin decondensation (NCD) was evaluated after exposure to a solution of sodium dodecyl sulfate/dithiothreitol. A decreased uptake of propidium iodide (PI), a DNA intercalating dye, was found in spermatozoa from the vas deferens of the lead-exposed mice. However, after thermal denaturation of the DNA, the spermatozoa showed a higher uptake of PI in comparison to those of the controls. After reductive cleavage of S-S bonds with DTT and staining with a thiol-specific reagent (monobromobimane), significantly fewer reactive disulfide bonds were also observed in the spermatozoa. Furthermore, a significant delay in the capacity for NCD was noted. These findings indicate that exposure to lead increases the stabilization of the sperm chromatin, which in turn probably affects the decondensation of the nucleus, thereby interfering with the fertility of the mice.