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Updated: Nov 1, 2025

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
Peptides Released from Extruded Adzuki Bean Protein through Simulated Gastrointestinal Digestion Exhibit
Zhenxing Shi1, Baoqing Dun1, Zuchen Wei1,2
1Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 80 South Xueyuan Road, Haidian District, Beijing 100081, People's Republic of China.
Abstract:
Increasing attention has been focused on plant-derived peptides because of their potential bioactivities. In this study, bioactive peptides were released from extruded adzuki bean protein by simulated gastrointestinal digestion. A peptide (KQS-1) sequenced as KQSESHFVDAQPEQQQR was separated and identified using ultrafiltration, pre-high-performance liquid chromatography (HPLC), and ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). KQS-1 was shown to exert significant anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing the production of IL-1, IL-6, TNF-α, and MCP-1 to 38.31, 6.07, 43.96, and 41.74%, respectively. The involved signaling pathways were identified by transcriptome analysis. Overall, 5236 differentially expressed genes (DEGs) were identified. Gene ontology (GO) functions demonstrated that DEGs were significantly related to the NF-κB pathway. In conclusion, KQS-1 prevented the activation and expression of NF-κB/caspase-1 by upstream and downstream factors. These findings highlight the bioactivity of adzuki bean peptides.
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