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Isoflurane induces liver injury by modulating the expression of miR-125a-5p
Xingwei Li1, Qun Yao1, Ruijun Li2
1Department of Anesthesiology, Zaozhuang Municipal Hospital, Zaozhuang, Shandong 277100, PR China.
Background:
Isoflurane can cause hepatotoxicity, and microRNAs (miRNAs) are involved in the regulation of liver injury. Therefore, this study aimed to explore the effect of miR-125a-5p on isoflurane-induced liver injury.
Basic Procedures:
Sprague-Dawley (SD) male rats and BRL-3A cells were exposed to isoflurane to construct animal and cell models. Serum alanine transaminase (ALT) and aspartate transaminase (AST) levels of rats were detected. RT-qPCR was performed for the measurement of miR-125a-5p levels. Cell proliferation and apoptosis were also detected.
Main Findings:
After isoflurane treatment, serum ALT and AST levels of rats increased in a time-dependent manner, and the differences reached significant levels from 3 days after isoflurane treatment. MiR-125a-5p levels increased significantly in the liver tissues of isoflurane-treated rats. MiR-125a-5p downregulation significantly attenuated isoflurane-induced increasing trend of serum ALT and AST levels in rats. In BRL-3A cells, isoflurane treatment significantly inhibited cell proliferation and promoted cell apoptosis, which was reversed by miR-125a-5p downregulation.
Principal Conclusions:
Isoflurane exposure significantly elevates the level of miR-125a-5p in the liver tissues of rats. Downregulation of miR-125a-5p may protect against isoflurane-induced liver injury through regulating liver cell proliferation and apoptosis. Dysregulation of miR-125a-5p might be a possible mechanism of isoflurane-induced liver injury.
Insights
Isoflurane causes liver injury, but downregulating microRNA-125a-5p (miR-125a-5p) may protect liver cells. This study found increased miR-125a-5p in isoflurane-induced liver damage, suggesting its role in hepatotoxicity.
Area of Science:
- Hepatology
- Molecular Biology
- Toxicology
Background:
- Isoflurane is known to cause hepatotoxicity.
- MicroRNAs (miRNAs) play a role in regulating liver injury.
- The specific role of miR-125a-5p in isoflurane-induced liver injury requires investigation.
Purpose of the Study:
- To investigate the effect of miR-125a-5p on isoflurane-induced liver injury.
- To explore the potential mechanism by which miR-125a-5p influences liver cell function under isoflurane exposure.
Main Methods:
- Construction of animal (Sprague-Dawley rats) and cell (BRL-3A) models of isoflurane-induced liver injury.
- Measurement of serum alanine transaminase (ALT) and aspartate transaminase (AST) levels.
- Quantification of miR-125a-5p levels using RT-qPCR.
- Assessment of cell proliferation and apoptosis.
Main Results:
- Isoflurane exposure increased serum ALT and AST levels in rats in a time-dependent manner.
- A significant increase in miR-125a-5p levels was observed in the liver tissues of isoflurane-treated rats.
- Downregulation of miR-125a-5p attenuated the isoflurane-induced increase in serum ALT and AST levels.
- In vitro, isoflurane inhibited cell proliferation and promoted apoptosis, effects reversed by miR-125a-5p downregulation.
Conclusions:
- Isoflurane exposure elevates miR-125a-5p levels in rat liver tissue.
- Downregulating miR-125a-5p may offer protection against isoflurane-induced liver injury by modulating hepatocyte proliferation and apoptosis.
- Dysregulation of miR-125a-5p is implicated as a potential mechanism in isoflurane-induced liver injury.
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