Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Testosterone: Functions and Regulation

Testosterone: Functions and Regulation

The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...

Aging

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Aging-related NADPH diaphorase positive neurodegenerations in the sacral spinal cord of aged non-human primates.

Scientific reports·2024

Same author

Edaravone Dexborneol ameliorates the cognitive deficits of APP/PS1 mice by inhibiting TLR4/MAPK signaling pathway via upregulating TREM2.

Neuropharmacology·2024

Same author

Testosterone deficiency worsens mitochondrial dysfunction in APP/PS1 mice.

Frontiers in aging neuroscience·2024

Same author

Single-cell and spatial transcriptomic investigation reveals the spatiotemporal specificity of the beta-defensin gene family during mouse sperm maturation.

Cell communication and signaling : CCS·2024

Same author

Retraction Note: Hedgehog signaling-related genomics signature for the accurate progress and prognosis prediction in gastric cancer.

Functional & integrative genomics·2024

Same author

Role of gut microbiota in doxorubicin-induced cardiotoxicity: from pathogenesis to related interventions.

Journal of translational medicine·2024

Search research articles

Related Experiment Video

Updated: Nov 1, 2025

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Published on: July 9, 2016

Testosterone ameliorates age-related brain mitochondrial dysfunction.

Wensheng Yan¹, Tianyun Zhang^1,2, Yunxiao Kang^1,2

¹Department of Neurobiology, Hebei Medical University, Shijiazhuang, China.

|June 17, 2021

Summary

Testosterone supplementation improves brain mitochondrial function and reduces oxidative damage in aging male rats, potentially preventing cognitive decline and neurodegenerative diseases.

Keywords:

aged male rats antioxidative capacity mitochondrial biogenesis mitochondrial function testosterone

More Related Videos

Orienteering as a Tool for Cognitive Research: An Implementation Guide

Orienteering as a Tool for Cognitive Research: An Implementation Guide

Published on: November 29, 2024

Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases

Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases

Published on: October 4, 2024

Related Experiment Videos

Last Updated: Nov 1, 2025

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Published on: July 9, 2016

Orienteering as a Tool for Cognitive Research: An Implementation Guide

Orienteering as a Tool for Cognitive Research: An Implementation Guide

Published on: November 29, 2024

Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases

Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases

Published on: October 4, 2024

Area of Science:

Neuroscience
Endocrinology
Gerontology

Background:

Aging in men is associated with brain mitochondrial dysfunction and reduced testosterone levels.
The relationship between these factors and the potential benefits of testosterone for brain health remains unclear.

Purpose of the Study:

To investigate whether testosterone supplementation can ameliorate mitochondrial dysfunction in the aging male brain.
To assess the impact of testosterone on cognitive-behavioral deficits and oxidative stress in aged male rats.

Main Methods:

Aging male rats were supplemented with testosterone.
Exploratory behavior, oxidative damage markers, mitochondrial function (membrane potential, enzyme activities, respiratory complexes), mitochondrial biogenesis markers (PGC-1α, NRF-1, TFAM), mitochondrial content (citrate synthase, mtDNA, gene expression), and mitophagy markers (PINK1/Parkin, P62) were assessed in the substantia nigra and hippocampus.

Main Results:

Testosterone supplementation alleviated behavioral deficits and oxidative damage in the brain.
Improved mitochondrial function, increased mitochondrial biogenesis and content, and activated mitophagy were observed.
Key indicators of mitochondrial health, including antioxidant enzyme activity and respiratory complex function, were enhanced.

Conclusions:

Testosterone supplementation effectively ameliorates brain mitochondrial dysfunction in aging male rats.
This suggests a potential therapeutic strategy for preventing or treating age-related cognitive-behavioral deficits and neurodegenerative conditions.