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Updated: Nov 1, 2025

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miR-1-3p/CELSR3 Participates in Regulating Malignant Phenotypes of Lung Adenocarcinoma Cells

Huiwen Miao1, Qingxin Zeng2, Shaohua Xu2

  • 1Department of Thoracic Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Current Gene Therapy
|June 18, 2021
PubMed
Abstract

Insights

This study reveals that miR-1-3p suppresses lung adenocarcinoma (LUAD) progression by targeting CELSR3. This finding offers new therapeutic strategies for LUAD patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Lung adenocarcinoma (LUAD) is a major cause of cancer-related mortality.
  • Understanding the molecular mechanisms driving LUAD progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of miR-1-3p in LUAD.
  • To identify potential therapeutic targets for LUAD treatment.

Main Methods:

  • Bioinformatics analysis of TCGA-LUAD data.
  • Quantitative real-time PCR (qRT-PCR) and Western blot for gene expression.
  • MTT and Transwell assays for cell phenotypes.
  • Luciferase reporter assays for miRNA-mRNA interaction verification.

Main Results:

  • miR-1-3p expression was decreased in LUAD.
  • Overexpression of miR-1-3p inhibited LUAD cell proliferation, migration, and invasion.
  • CELSR3, a direct target of miR-1-3p, was upregulated in LUAD and promoted malignant phenotypes.
  • CELSR3 overexpression reversed the inhibitory effects of miR-1-3p on LUAD cells.

Conclusions:

  • miR-1-3p suppresses LUAD cell malignant phenotypes by targeting CELSR3.
  • This miR-1-3p/CELSR3 axis presents a novel therapeutic strategy for LUAD.
Keywords:
CELSR3Lung adenocarcinomainvasion.miR-1-3pmigrationproliferation

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