Toxicological assessment of CeO₂ nanoparticles on early development of zebrafish

Nanomaterials involve an active research and a booming area including different fields (health, environment, electronics, manufacturing, drug delivery). Recently, new concerns are emerging about the risk from increased production and subsequent release into the environment, as they are largely present in consumer products and industrial applications. Our aim was to assess the effects of three different types of cerium oxide nanoparticles (CeO₂ NPs) (type 1 defined "as prepared"; type 2 defined "modified"; type 3 defined "commercial") on zebrafish embryos by Fish Embryo Toxicity test (Z-FET). Immunohistochemical analysis was also performed on treated larvae to evaluate the expression of the following biomarkers: Metallothionein, Heat Shock Protein 70 (HSP70) and 7-Ethoxyresorufin-O-Dietylase (EROD). After 96 h of exposure, there was no lethality, nor were there sub-lethal effects in embryonic development, when compared with the control. No particular positivity was found about Metallothionein and HSP70 expression, while an increased expression of EROD was observed in larvae exposed to the three types of CeO₂ NPs compared with the controls. The analyze has confirmed a statistically significant difference (P < 0.001) to EROD biomarker between control group and treated larvae response, finding was higher at 1-mg/l concentration. Further investigations in order to solve conflicting views about potential effects of CeO₂ NPs are necessary, also to evaluate its effectiveness in different fields as already reported in literature.