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Related Experiment Video

Updated: Nov 1, 2025

Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
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Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

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Protocol for tissue slice cultures from human solid tumors to study therapeutic response.

Heidi L Kenerson1, Kevin M Sullivan1, Kevin P Labadie1

  • 1University of Washington Department of Surgery, Seattle, WA 98195, USA.

STAR Protocols
|June 18, 2021
PubMed
Summary

This study introduces a flexible slice culture protocol to analyze systemic therapy effects on the tumor microenvironment in gastrointestinal cancers. This method enables detailed investigation of treatment responses using various analyses.

Keywords:
CancerCell cultureCell-based AssaysHigh Throughput ScreeningImmunology

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Area of Science:

  • Oncology
  • Cancer Biology
  • Translational Medicine

Background:

  • Studying systemic therapy's impact on the tumor microenvironment in human solid tumors is challenging.
  • Existing methods may not fully capture complex tumor-host interactions.
  • A need exists for robust models to predict therapeutic efficacy.

Purpose of the Study:

  • To present a novel protocol for establishing human solid tumor slice cultures.
  • To enable the investigation of responses to various systemic therapies, including chemotherapies, immunotherapies, and adoptive cell therapies.
  • To provide a versatile platform for multi-endpoint analyses of treatment effects.

Main Methods:

  • Establishment of human solid tumor slice cultures from patient-derived samples.
  • Application of diverse systemic therapies (chemotherapy, immunotherapy, cell therapy) to slice cultures.
  • Comprehensive analysis of treatment response using viability assays, histology, live-cell imaging, and multi-omics.

Main Results:

  • The protocol is applicable to a wide range of gastrointestinal malignancies.
  • Demonstrated feasibility of assessing treatment-induced changes in tumor viability and histology.
  • Enabled detailed characterization of cellular and molecular responses through advanced imaging and multi-omics.

Conclusions:

  • The developed slice culture protocol offers a flexible and adaptable platform for studying systemic therapy effects on the tumor microenvironment.
  • This model facilitates the preclinical evaluation of various cancer treatments in a human-relevant context.
  • The protocol supports multi-modal analyses, providing deep insights into treatment response and resistance mechanisms.