Nocturnal stomatal conductance in wheat is growth-stage specific and shows genotypic variation

Nocturnal stomatal conductance (g_sn ) represents a significant source of water loss, with implications for metabolism, thermal regulation and water-use efficiency. With increasing nocturnal temperatures due to climate change, it is vital to identify and understand variation in the magnitude and responses of g_sn in major crops. We assessed interspecific variation in g_sn and daytime stomatal conductance (g_s ) in a wild relative and modern spring wheat genotype. To investigate intraspecific variation, we grew six modern wheat genotypes and two landraces under well watered, simulated field conditions. For the diurnal data, higher g_sn in the wild relative was associated with significantly lower nocturnal respiration and higher daytime CO₂ assimilation while both species exhibited declines in g_sn post-dusk and pre-dawn. Lifetime g_sn achieved rates of 5.7-18.9% of g_s . Magnitude of g_sn was genotype specific 'and positively correlated with g_s . g_sn and g_s were significantly higher on the adaxial surface. No relationship was determined between harvest characteristics, stomatal morphology and g_sn , while cuticular conductance was genotype specific. Finally, for the majority of genotypes, g_sn declined with age. Here we present the discovery that variation in g_sn occurs across developmental, morphological and temporal scales in nonstressed wheat, presenting opportunities for exploiting intrinsic variation under heat or water stressed conditions.