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Related Experiment Video

Updated: Nov 1, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Nitric Oxide Photoreleasers with Fluorescent Reporting.

Aurore Fraix¹, Cristina Parisi¹, Mimimorena Seggio¹

¹PhotoChemLab, Department of Drug and Health Sciences, University of Catania, 95125, Catania, Italy.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|June 18, 2021

Summary

Photoactivatable nitric oxide (NO) releasers offer controlled NO delivery for therapeutics. These compounds enable real-time, non-invasive tracking of NO release using fluorescence, enhancing therapeutic potential.

Keywords:

fluorescence imaging light nitric oxide therapy

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Last Updated: Nov 1, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Area of Science:

Biomedical Engineering
Chemical Biology
Photopharmacology

Background:

Nitric oxide (NO) is crucial in physiology and pathophysiology.
Controlled NO delivery is vital for therapeutic applications.
Current methods lack precise control over NO release and monitoring.

Purpose of the Study:

To review advances in photoactivatable NO releasers with fluorescent reporting.
To highlight the design rationale for these molecular constructs.
To discuss their potential therapeutic applications.

Main Methods:

Development of molecular constructs for light-activated NO release.
Integration of fluorescent reporting functionalities for real-time NO tracking.
In vitro and in vivo studies to evaluate NO release and biological effects.

Main Results:

Successful design and synthesis of photoactivatable NO donors.
Demonstration of light-triggered NO release with spatial and temporal control.
Concomitant fluorescence reporting accurately quantifies NO release in cellular environments.

Conclusions:

Photoactivatable NO releasers offer precise control over NO delivery.
Fluorescent reporting enables non-invasive monitoring of NO release.
These advanced compounds hold significant promise for future therapeutic strategies.