Performance and microbial community dynamics relationship within a step-feed anoxic/oxic/anoxic/oxic process...

A step-feed anoxic/oxic/anoxic/oxic (SF-A/O/A/O) was developed and successfully applied to full-scale coking wastewater treatment. The performance and microbial community were evaluated and systematically compared with the anoxic/oxic/oxic (A/O/O) process. SF-A/OA/O process exhibited efficient removal of COD, NH₄⁺-N, TN, phenols, and cyanide with corresponding average effluent concentrations of 317.9, 1.8, 46.2, 1.1, and 0.2 mg·L^-1, respectively. In particular, the TN removal efficiency of A/O/O process was only 7.8%, with an effluent concentration of 300.6 mg·L^-1. Furthermore, polycyclic aromatic hydrocarbons with high molecular weight were the dominant compounds in raw coking wastewater, which were degraded to a greater extent in SF-A/OA/O. The abundance in Thiobacillus, SM1A02, and Thauera could be the main reason why SF-A/O/A/O was superior to A/O/O in treating TN. The microbial community structure of SF-A/O/A/O was similar among stages in system (P ≥ 0.05, Welch's t-test) and was less affected by environmental factors, which may have been one of the important factors in the system's strong stability.