Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

12.3K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
12.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.8K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.8K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.4K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gastric pH-induced liquid-liquid phase separation between lactoferrin and osteopontin protects against proteolysis and modulates intestinal uptake.

Food research international (Ottawa, Ont.)·2026
Same author

Metabolic impact of reduced-protein Nordic diet-based complementary feeding: a secondary analysis of a randomized controlled study.

The American journal of clinical nutrition·2026
Same author

Intelectin-2 is a broad-spectrum antimicrobial lectin.

Nature communications·2026
Same author

Infant formula iron fortification of 2 vs. 8 mg/L does not increase the risk of iron deficiency or impact neurodevelopment at 12 months.

Journal of pediatric gastroenterology and nutrition·2025
Same author

Impact of bovine lactoferrin supplementation and reduced iron in formula on infant oral microbiome: a randomized controlled trial.

Journal of oral microbiology·2025
Same author

Assessment of the protective effects of milk osteopontin in necrotizing enterocolitis neonatal rat model.

The Journal of nutritional biochemistry·2025

Related Experiment Video

Updated: Nov 1, 2025

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

1.1K

Human intelectin-1 (ITLN1) genetic variation and intestinal expression.

Eric B Nonnecke1, Patricia A Castillo2,3, Amanda E Dugan4

  • 1Department of Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, 95616, USA. ebnonnecke@ucdavis.edu.

Scientific Reports
|June 19, 2021
PubMed
Summary

Intelectin 1 (ITLN1) gene variants linked to Crohn's disease (CD) risk do not appear to alter ITLN1 expression or function in the gut. The CD risk may stem from other genes like CD244 or Ly9.

More Related Videos

Innervation of Human Intestinal Organoids
07:23

Innervation of Human Intestinal Organoids

Published on: January 17, 2025

786
Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids
08:13

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

2.5K

Related Experiment Videos

Last Updated: Nov 1, 2025

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

1.1K
Innervation of Human Intestinal Organoids
07:23

Innervation of Human Intestinal Organoids

Published on: January 17, 2025

786
Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids
08:13

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

2.5K

Area of Science:

  • Immunology
  • Genetics
  • Molecular Biology

Background:

  • Intelectins are ancient carbohydrate-binding proteins involved in human diseases.
  • Genome-wide association studies (GWAS) have associated single nucleotide polymorphisms (SNPs) in ITLN1 with Crohn's disease (CD) susceptibility.
  • The functional impact of these ITLN1 locus SNPs remains unclear.

Purpose of the Study:

  • To investigate the functional significance of CD-associated SNPs at the ITLN1 locus.
  • To determine if these SNPs affect ITLN1 expression, glycan binding, or protein oligomerization in intestinal tissue.
  • To explore potential alternative genetic explanations for the observed CD risk.

Main Methods:

  • Linkage disequilibrium (LD) analysis using Ensembl database to identify associated SNPs.
  • Quantification of ITLN1 mRNA and protein levels in intestinal tissue from CD patients.
  • Assessment of the V109D missense variant's effect on ITLN1 glycan binding and oligomerization.

Main Results:

  • Several disease-associated SNPs at the ITLN1 locus, including those in CD244 and Ly9, were found to be in LD.
  • ITLN1 mRNA and protein levels in intestinal tissue were not altered in CD samples or by genotype.
  • The V109D variant did not affect ITLN1's glycan binding or protein-protein oligomerization.

Conclusions:

  • The identified CD-risk haplotype is unlikely to confer risk through altered ITLN1 carbohydrate recognition, protein oligomerization, or expression in the intestinal mucosa.
  • The association between the genomic data and CD may be due to functional changes in CD244 or Ly9.
  • Alternative explanations include altered ITLN1 expression in other tissues or modified protein interactions.