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Updated: Nov 1, 2025

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
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Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

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Serum microRNA-33 levels in pre-diabetic and diabetic patients

Beydolah Shahouzehi1, Mostafa Eghbalian2, Hossein Fallah3

  • 1Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.

Molecular Biology Reports
|June 19, 2021
PubMed

Insights

Serum miR-33 levels are elevated in pre-diabetic individuals, suggesting its potential role in monitoring pre-diabetes. Further research is needed to confirm its diagnostic viability.

Area of Science:

  • Biochemistry
  • Genetics
  • Endocrinology

Background:

  • Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia.
  • MicroRNAs (miRNAs) are small non-coding RNAs with regulatory roles in cellular functions and disease biomarkers.
  • Dysregulation of miRNAs is implicated in the pathogenesis of DM.

Purpose of the Study:

  • To investigate the expression levels of miR-33 in non-diabetic, pre-diabetic, and diabetic individuals.
  • To explore the correlation between serum miR-33 levels and metabolic parameters associated with diabetes.
  • To assess the potential of miR-33 as a biomarker for pre-diabetes.

Main Methods:

  • Serum samples were collected from three groups: non-diabetic controls, pre-diabetic individuals, and DM patients (n=15 per group).
  • Quantitative real-time PCR was employed to measure serum miR-33 expression levels.
  • Statistical analyses were performed to determine correlations between miR-33 and clinical/biochemical parameters.

Main Results:

  • Serum miR-33 expression was significantly higher in the pre-diabetic group compared to non-diabetic and diabetic groups (p < 0.001).
  • Diabetic subjects exhibited elevated fasting blood sugar (FBS), insulin levels, HOMA-IR, and triglycerides (TG).
  • miR-33 positively correlated with pre-diabetic state, sex, and FBS, and negatively with HOMA-IR.

Conclusions:

  • Elevated serum miR-33 levels are associated with the pre-diabetic state.
  • miR-33 may serve as a potential biomarker for monitoring the onset and progression of pre-diabetes.
  • Larger-scale studies are warranted to elucidate the precise mechanism and diagnostic utility of miR-33 in diabetes.
Keywords:
Diabetes mellitusInsulinReal-time PCRmiR-33

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