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Updated: Nov 1, 2025
08:23
High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
Published on: August 19, 2025
336
Cell death due to electroporation - A review
Tina Batista Napotnik1, Tamara Polajžer1, Damijan Miklavčič1
1University of Ljubljana, Faculty of Electrical Engineering, Tržaška cesta 25, 1000 Ljubljana, Slovenia.
Bioelectrochemistry (Amsterdam, Netherlands)
|June 19, 2021
Summary
Electroporation uses electric pulses to alter cell membranes for treatments like gene therapy or tumor ablation. Understanding cell death mechanisms is key to optimizing these medical and biotechnological applications.
Area of Science:
- Biophysics
- Cell Biology
- Biotechnology
Background:
- High voltage electric pulses induce membrane electroporation, increasing cell permeability.
- Electroporation can be reversible (gene/drug delivery) or irreversible (tissue ablation).
- Mechanisms of cell death and membrane repair following electroporation are not fully understood.
Purpose of the Study:
- To review cell death mechanisms after electroporation.
- To identify cell injuries leading to cell death post-electroporation.
- To explore membrane repair mechanisms involved in electroporation.
Main Methods:
- Literature review of studies on electroporation and cell death.
- Analysis of reported cell injuries and repair mechanisms.
- Identification of cellular targets affected by electric fields.
Main Results:
- Electroporation's effects on cell viability depend on pulse parameters and conditions.
- Specific cell death pathways and membrane repair processes are influenced by electroporation.
- Understanding these mechanisms is crucial for treatment efficacy.
Conclusions:
- Further research into cell death and membrane repair mechanisms is needed.
- Identifying electric field targets within cells is essential.
- Optimizing electroporation techniques requires deeper knowledge of cellular responses to electric pulses.
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