Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

10.2K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.2K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

5.1K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.1K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.4K
RNA Interference01:23

RNA Interference

26.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.9K
Enzyme Inhibition01:30

Enzyme Inhibition

87.3K
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
87.3K
Experimental RNAi02:15

Experimental RNAi

6.6K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nomogram for Preoperative Prediction of Adjuvant Therapy Requirement Following Radical Surgery in Stage IB Cervical Squamous Cell Carcinoma With Tumor Size ≤ 4 cm: A Retrospective Study.

Cancer medicine·2026
Same author

[Influence of nutritional composition and digestibility of different oat products on their glycemic index].

Wei sheng yan jiu = Journal of hygiene research·2026
Same author

Nanopore sequencing with proteins: synchronization and dischronization of molecular dynamics simulations with laboratory and industrial developments.

Soft matter·2026
Same author

NanoporeDB: A Structural Resource Of Multimeric Protein Nanopores For Single-Molecule Sensing.

GigaScience·2026
Same author

The skull bone marrow-meninges-brain axis: A new sentinel for neuroinflammation regulation in ischemic stroke.

Experimental neurology·2026
Same author

Precision glycoengineered AAV capsids enhance hepatocyte targeting and attenuated immune activation for liver-directed gene therapy.

Biomaterials·2026

Related Experiment Video

Updated: Nov 1, 2025

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

6.8K

Tenovin-1 inhibited dengue virus replication through SIRT2.

Yihong Wan1, Wenyu Wu2, Jiawen Zhang1

  • 1State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, PR China.

European Journal of Pharmacology
|June 20, 2021
PubMed
Summary

Tenovin-1, a SIRT inhibitor, effectively combats dengue virus (DENV) by reducing viral replication and inflammation. This compound shows promise as a new antiviral treatment for flavivirus infections.

Keywords:
Dengue feverSIRT2SirtuinsTenovin-1

More Related Videos

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

32.2K
Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors
10:29

Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors

Published on: May 9, 2025

1.7K

Related Experiment Videos

Last Updated: Nov 1, 2025

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

6.8K
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

32.2K
Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors
10:29

Quantitative Structure-Activity Relationship, Activity Prediction, and Molecular Dynamics of Non-nucleotide Reverse Transcriptase Inhibitors

Published on: May 9, 2025

1.7K

Area of Science:

  • Virology
  • Pharmacology
  • Immunology

Background:

  • Dengue fever is a widespread arbovirus disease with no effective treatments or safe vaccines.
  • Sirtuins (SIRTs) are implicated in dengue virus infection.
  • There is an urgent need for novel antiviral agents against dengue virus.

Purpose of the Study:

  • To investigate the antiviral potential of tenovin-1, a SIRT1/2 inhibitor, against dengue virus (DENV).
  • To elucidate the mechanism of action of tenovin-1 in inhibiting DENV replication and associated inflammatory responses.

Main Methods:

  • In vitro antiviral assays using BHK-21 cells infected with DENV.
  • Drug-addition assays to determine the stage of antiviral action.
  • Measurement of viral protein and mRNA levels.
  • Assessment of inflammatory cytokine release.
  • Experiments involving SIRT agonists and SIRT2 knockdown.

Main Results:

  • Tenovin-1 demonstrated significant antiviral activity against all four DENV serotypes in vitro, with EC50 values ranging from 0.97 to 3.81 μM.
  • Tenovin-1 treatment reduced viral cytopathic effects, inhibited progeny virus release, and decreased viral protein and mRNA levels.
  • The compound acts post-viral infection and reduces inflammatory responses by lowering cytokine release.
  • The antiviral effect was confirmed to be on-target, as it was abrogated by SIRT agonists or SIRT2 knockdown.

Conclusions:

  • Tenovin-1 exhibits potent antiviral activity against dengue virus through inhibition of SIRT2.
  • Tenovin-1 also modulates the inflammatory response during DENV infection.
  • This compound represents a promising therapeutic candidate for flavivirus infections and warrants further investigation.