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The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium
Robert P De Poi1, Michael Kowolik2, Yoshiki Oshida3
1Division of Dentistry, Medicine and Health Science, University of Melbourne, Melbourne, VIC, Australia.
Frontiers in Immunology
|June 21, 2021
Summary
Titanium surfaces prime monocytes for enhanced respiratory burst activity after 60-minute incubation. Surface energy significantly impacts this cellular response, crucial for osseointegration and healing.
Area of Science:
- Biomaterials Science
- Cellular Biology
- Immunology
Background:
- Osseointegration success relies on cellular responses to implanted biomaterials.
- Monocyte activation is critical for the inflammatory and healing phases post-implantation.
Purpose of the Study:
- To investigate the in vitro priming and activation of monocyte respiratory burst activity.
- To assess the influence of surface-modified titanium (grade 2 commercially pure titanium - CPT) with varying surface energies and roughness on monocyte activation.
Main Methods:
- Human peripheral blood monocytes were incubated with surface-modified CPT disks (30 or 60 min).
- Respiratory burst activity was measured using luminol-enhanced chemiluminescence (LCL) after secondary stimulation with phorbol 12-myrisate 13-acetate (PMA).
- Surface morphology was analyzed using scanning electron microscopy (SEM).
Main Results:
- A 60-minute incubation with titanium primed monocytes, enhancing respiratory burst activity.
- A 30-minute incubation resulted in reduced LCL compared to control cells.
- The titanium disk with the lowest surface energy consistently produced significantly less LCL.
Conclusions:
- Titanium surface characteristics, particularly surface energy, influence monocyte priming and activation.
- These findings highlight the importance of surface modification in regulating cellular responses for improved osseointegration.
