Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction

Area of Science:

• Veterinary Medicine
• Immunology
• Pathogen Research

Background:

• Advancements in host-pathogen interaction analysis necessitate sophisticated tissue models.
• Existing models like organoids and organs-on-a-chip lack the complexity of native host tissue.
• Differences between mouse and other mammal models highlight the need for more relevant systems.

Purpose of the Study:

• To review the current literature on precision cut tissue slices (PCTS) in veterinary species.
• To advocate for PCTS as valuable tools for studying host-pathogen interactions and vaccine responses.
• To highlight the potential of PCTS in advancing precision medicine approaches in veterinary health.

Main Methods:

• Review of existing scientific literature on PCTS in veterinary research.
• Discussion of the capabilities of PCTS in modeling complex host-pathogen interactions.
• Comparison of PCTS with other tissue models like organoids and organs-on-a-chip.

Main Results:

• PCTS provide a "whole organ" ex-vivo model that captures cellular and structural complexity.
• PCTS are suitable for assessing host-pathogen interactions and vaccine responses.
• PCTS models can bridge the gap between target identification and clinical translation.

Conclusions:

• Precision cut tissue slices (PCTS) are remarkable tools for ex-vivo analysis of host-pathogen interactions and vaccine responses in veterinary species.
• PCTS offer a more relevant model compared to current organoids and organs-on-a-chip technologies.
• The adoption of PCTS can accelerate the translation of research findings into clinical applications and precision medicine.