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Related Experiment Video

Updated: Nov 1, 2025

Methods for Intravenous Self Administration in a Mouse Model
12:09

Methods for Intravenous Self Administration in a Mouse Model

Published on: December 8, 2012

54.3K

Operant Vapor Self-administration in Mice.

Renata C N Marchette1, Brendan J Tunstall2, Leandro F Vendruscolo1

  • 1Intramural Research Program, National Institute for Drug Abuse, National Institutes of Health, Baltimore, MD, USA.

Bio-Protocol
|June 21, 2021
PubMed
Summary

Researchers developed a novel, non-invasive mouse model for studying opioid addiction using vaporized fentanyl. This method overcomes limitations of traditional models, enabling extended research into addiction behaviors and neurobiology.

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Addiction Research

Background:

  • Rodent models are crucial for understanding drug addiction neurobiology.
  • Intravenous drug self-administration in mice is common but faces challenges like catheter patency.
  • Existing models limit long-term studies and integration with advanced techniques.

Purpose of the Study:

  • To develop a non-invasive mouse model for opioid self-administration.
  • To overcome the limitations of intravenous self-administration models.
  • To facilitate the study of opioid addiction using genetic tools and advanced techniques.

Main Methods:

  • Developed a non-invasive mouse model utilizing vaporized fentanyl for self-administration.
  • The model allows for the study of various addiction-related behaviors.
Keywords:
FentanylMouse modelOpioid addictionOpioid use disorderSelf-administrationVapor

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Related Experiment Videos

Last Updated: Nov 1, 2025

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  • Bypasses complications associated with intravenous catheterization.

    • Main Results:

    • The vaporized fentanyl model enables the study of self-administration, escalation, extinction, and reinstatement.
    • This non-invasive approach allows for extended drug-taking investigations.
    • Facilitates integration with techniques like calcium imaging and in vivo electrophysiology.

    Conclusions:

    • The non-invasive vaporized fentanyl model offers a significant advancement for opioid addiction research in mice.
    • It overcomes key limitations of current intravenous models, enabling more comprehensive studies.
    • This model supports the use of powerful genetic and physiological tools to investigate opioid addiction.