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Collection and preprocessing of fine needle aspirate patient samples for single cell profiling and data analysis
Aizhan Tastanova1, Egle Ramelyte1, Zsolt Balázs2,3
1Dermatology Department, University Hospital Zurich and Medical Faculty, University of Zurich, 8091 Zurich, Switzerland.
STAR Protocols
|June 21, 2021
Abstract
None:
High cell viability and recovered cell concentration are typical quality control requirements for single-cell processing and quality data. This protocol describes procedures for sampling, live-cell biobanking, preprocessing for single-cell RNA sequencing, and analysis of fine-needle aspiration (FNA) samples of the skin. The minimally invasive nature of FNA collection is more accepted by patients and allows for frequent longitudinal sampling, resulting in high-quality single-cell sequencing data that capture cellular heterogeneity in clinical samples.