PUMA - An open-source 3D-printed direct vision microscope with augmented reality and spatial light modulator...

3D-printed microscopes are a topical emerging field in the literature. However most microscopes presented to date are quite novel re-imaginings of the microscope's mechanical design and they are either solely dependent on, or primarily geared towards, camera-based observations rather than ergonomic direct vision screening through an ocular lens. The reliance on camera, computer and monitor for observation introduces a compromise between portability, cost and the quality of an instant wide field of view. In this report, I introduce the Portable Upgradeable Modular and Affordable (PUMA) microscope which is an open-source 3D-printed multimodality microscope that employs a traditional upright design for ease of human direct visual observations and slide screening. PUMA uses standard RMS or C-mount objectives, with a tube length 160 mm, 170 mm or infinity and wide field high eye point ocular lenses. PUMA can use simple mirror-based illumination or can be configured to a full Köhler system with Abbe condenser for high numerical aperture observations including oil immersion. PUMA also has advanced digital/optical imaging features such as a digital spatial light modulator and - unique to any 3D printed microscope to date - an augmented reality heads-up display for interactive calibrated measurements. Digital camera imaging can also be used with PUMA - in fact PUMA can take up to three separate digital cameras simultaneously. PUMA can also function as a direct vision multi-header microscope for teaching or discussion. The illumination system is also modular and includes transillumination, epi-illumination, fluorescence, polarisation, dark ground and also Schlieren-based phase contrast and other Fourier optics filtering modalities. All these advanced features are available through an on-board, battery operated, microprocessor so no mains supply, smartphone, network connection, PC or external monitor are required making PUMA a truly portable system suitable for remote field work.