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Related Experiment Video

Updated: Nov 1, 2025

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

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Published on: December 9, 2017

Catalytic function, mechanism, and application of plant acyltransferases.

Linlin Wang¹, Kuan Chen¹, Meng Zhang¹

¹State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.

Critical Reviews in Biotechnology

|June 21, 2021

Summary

Acyltransferases (ATs) are key enzymes modifying natural products. This review details 141 plant-derived ATs, focusing on their functions, expression, and catalytic mechanisms for potential applications.

Keywords:

BAHD-AT SCPL-AT catalytic application natural products plant acyltransferase

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Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

Published on: June 20, 2019

Area of Science:

Biochemistry
Natural Product Chemistry
Enzymology

Background:

Acyltransferases (ATs) are crucial enzymes in natural product biosynthesis.
They modify compounds, enhancing properties like lipid solubility and pharmacological activity.
Plant-derived ATs are increasingly important for natural product diversity.

Purpose of the Study:

To review and summarize biochemically characterized acyltransferases (ATs) from plants.
To consolidate information on their functions, expression systems, and catalytic mechanisms.
To discuss the catalytic performance and application potential of these enzymes.

Main Methods:

Literature review of studies published between July 1997 and October 2020.
Inclusion of 141 biochemically characterized plant acyltransferases.
Analysis of enzyme function, heterologous expression, and catalytic mechanisms.

Main Results:

Compilation of 141 characterized plant acyltransferases.
Detailed summary of their diverse functions and catalytic mechanisms.
Assessment of their heterologous expression systems and performance.

Conclusions:

Acyltransferases play a significant role in natural product structural and functional diversity.
Understanding their mechanisms and expression is key to harnessing their potential.
These enzymes offer promising applications in biotechnology and drug discovery.