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Syncollin is an antibacterial polypeptide.

Rosie A Waters1, James Robinson2, J Michael Edwardson1

  • 1Department of Pharmacology, University of Cambridge, Cambridge, UK.

Cellular Microbiology
|June 21, 2021
PubMed
Summary
This summary is machine-generated.

Syncollin, a pancreatic protein, exhibits potent antimicrobial activity by binding to bacterial peptidoglycan and damaging bacterial membranes. This finding suggests syncollin

Keywords:
antibacterial polypeptidepancreatic zymogen granulepeptidoglycansyncollin

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Area of Science:

  • Microbiology
  • Biochemistry
  • Immunology

Background:

  • Syncollin is a 16-kDa protein primarily located in pancreatic zymogen granules.
  • Its presence in intestinal epithelial cells and neutrophils suggests a potential role in host defense.

Purpose of the Study:

  • To investigate the antimicrobial properties of syncollin.
  • To determine if syncollin plays a role in host defense mechanisms within the gut.

Main Methods:

  • Isolation of Strep-tagged syncollin from transfected mammalian cells.
  • Circular dichroism spectroscopy to analyze protein structure.
  • Bacterial growth inhibition assays with Lactococcus lactis and Escherichia coli.
  • Propidium iodide uptake assays to assess membrane permeability.
  • Scanning electron microscopy to visualize bacterial surface damage.

Main Results:

  • Syncollin is exceptionally thermostable with a predominantly beta-sheet structure.
  • Syncollin binds to bacterial peptidoglycan, inhibiting the growth of both Gram-positive and Gram-negative bacteria.
  • Syncollin induces membrane permeabilization in E. coli and causes surface structural damage in both E. coli and L. lactis.

Conclusions:

  • Syncollin possesses significant antimicrobial properties.
  • Syncollin likely functions as a component of the innate immune system, contributing to the control of the gut microbiome.